Microfluidic chip reaction chambers are core components of precision in vitro detection, with internal micro-channel width as low as 20μm, requiring ultra-high welding precision, no blockage, no contamination, ensuring smooth fluid flow and accurate reaction. Traditional assembly processes easily block micro-channels or generate particles, leading to detection failure. DaokeDao laser welding has ultra-high positioning accuracy (±0.003mm), with concentrated laser energy, heat-affected zone ≤0.15mm, completely avoiding micro-channel blockage and deformation, preserving the complete structure of internal micro-channels. The process is suitable for medical-grade COC and PDMS materials, particle-free and residue-free, no contamination of detection samples, fully meeting microfluidic detection cleanliness and biocompatibility requirements. The weld has high transparency, facilitating real-time observation of sample reaction process. The equipment supports small-batch high-precision customized production, adapting to different microfluidic chip designs, helping diagnostic equipment manufacturers improve detection accuracy and develop high-end precision diagnostic products.